Seasonal variation in Blueberry scorch virus concentration in highbush blueberry and implications for disease monitoring and management

Seasonal variation in relative Blueberry scorch virus (BlScV) concentration was determined for three infected highbush blueberry, Vaccinium corymbosum, cultivars in a commercial field in southwestern British Columbia, Canada. Relative virus concentration per g of infected blueberry flower clusters and leaf terminal tissue varied during the season with significant cultivar-by-time interactions. Flower clusters had the highest BlScV concentration per g of tissue and could be collected in early May for disease surveys. Timing of leaf sample collection for BlScV surveys, transmission studies and virus purification should be based on studies of temporal variation in BlScV concentration for the principal cultivars in a production area.Les variations saisonnières dans la concentration relative du virus de la brunissure nécrotique du bleuet (Blueberry scorch virus; BlScV) ont été déterminées pour trois cultivars infectés du bleuetier géant, Vaccinium corymbosum, dans un champ commercial du sud-ouest de la Colombie-Britannique, Canada. La concentration relative du virus par g de grappes de fleurs et de tissu terminal de feuille infectés a varié au cours de la saison, avec des interactions significatives entre les cultivars et le temps. Les grappes de fleurs avaient la plus haute concentration du virus par g de tissu et pouvaient être cueillies au début de mai pour évaluer la maladie. Le moment choisi pour la collecte des échantillons de feuille pour l’étude du BlScV, les études de transmission et le traitement du virus devrait se fonder sur des études de variation temporelle dans la concentration du BlScV pour les principaux cultivars d’une zone de production

Sparsity-based autoencoders for denoising cluttered radar signatures

Narrowband and broadband indoor radar images significantly deteriorate in the presence of target-dependent and target-independent static and dynamic clutter arising from walls. A stacked and sparse denoising autoencoder (StackedSDAE) is proposed for mitigating the wall clutter in indoor radar images. The algorithm relies on the availability of clean images and the corresponding noisy images during training and requires no additional information regarding the wall characteristics. The algorithm is evaluated on simulated Doppler-time spectrograms and high-range resolution profiles generated for diverse radar frequencies and wall characteristics in around-the-corner radar (ACR) scenarios. Additional experiments are performed on range-enhanced frontal images generated from measurements gathered from a wideband radio frequency imaging sensor. The results from the experiments show that the StackedSDAE successfully reconstructs images that closely resemble those that would be obtained in free space conditions. Furthermore, the incorporation of sparsity and depth in the hidden layer representations within the autoencoder makes the algorithm more robust to low signal-to-noise ratio (SNR) and label mismatch between clean and corrupt data during training than the conventional single-layer DAE. For example, the denoised ACR signatures show a structural similarity above 0.75 to clean free space images at SNR of −10 dB and label mismatch error of 50%

Pre-main-sequence population in NGC 1893 region: X-ray properties

Continuing the attempt to understand the properties of the stellar content in the young cluster NGC 1893 we have carried out a comprehensive multi-wavelength study of the region. The present study focuses on the X-ray properties of T-Tauri Stars (TTSs) in the NGC 1893 region. We found a correlation between the X-ray luminosity, $L_X$, and the stellar mass (in the range 0.2$-$2.0 \msun) of TTSs in the NGC 1893 region, similar to those reported in some other young clusters, however the value of the power-law slope obtained in the present study ($\sim$ 0.9) for NGC 1893 is smaller than those ($\sim$1.4 - 3.6) reported in the case of TMC, ONC, IC 348 and Chameleon star forming regions. However, the slope in the case of Class III sources (Weak line TTSs) is found to be comparable to that reported in the case of NGC 6611 ($\sim$ 1.1). It is found that the presence of circumstellar disks has no influence on the X-ray emission. The X-ray luminosity for both CTTSs and WTTSs is found to decrease systematically with age (in the range $\sim$ 0.4 Myr - 5 Myr). The decrease of the X-ray luminosity of TTSs (slope $\sim$ -0.6) in the case of NGC 1893 seems to be faster than observed in the case of other star-forming regions (slope -0.2 to -0.5). There is indication that the sources having relatively large NIR excess have relatively lower $L_X$ values. TTSs in NGC 1893 do not follow the well established X-ray activity - rotation relation as in the case of main-sequence stars.Comment: 10 pages, 7 figures, Accepted for publication in New Astronom

Ras hyperactivation versus overexpression : Lessons from Ras dynamics in Candida albicans

We thank Prof. Neta Dean for the CIp10ADH1-Cherry plasmid and Prof. Aaron Mitchell for the BWP17 strain. We gratefully acknowledge Prof. Sudipta Maiti, TIFR, Mumbai, India for providing the data acquisition software. We also appreciate the feedback and discussions with Dr. Rohini Muthuswami, SLS, JNU as well as from the Protein Society group, New Delhi while this study was taking shape. We thank Prof. Alok Bhattacharya for Cytochalasin D. The GC-MS and fluorescence lifetime measurements were carried out at the Advanced Instrumentation Research Facility (AIRF), JNU. Confocal images were recorded either at the central instrumentation facility (CIF), SLS, JNU or at AIRF, JNU. This work was supported by project grants from Department of Biotechnology (DBT, Project grant no. BT/PR20410/BRB/10/1542/2016) and Department of Science and Technology (DST, Project grant no. SB/SO/BB-011/2014), India to S.S.K; and project grants from Department of Information Technology, (DIT, Project grant no. 12(4)/2007-PDD), India to S.S. for FCS setup. In addition, both S.S. and S.S.K. thank DBT-BUILDER for funding support (Project grant no. BT/PR5006/INF/153/2012). S.S.K. also acknowledges funding support from UGC Resource Networking grant to the School of Life Sciences. We thank DST-PURSE and JNU for assistance with funding for publication. G.S.V. and S.C.S. received a fellowship from UGC; V.A.P., B.Y., P.J., N.P., M.F.K. acknowledge CSIR for fellowships. S.L.S. received a fellowship from ICMR. D.T.H. and M.F.K. thank DBT-BUILDER for funding.Peer reviewedPublisher PD

CCD photometry of the unstudied galactic star clusters Be 10, Be 67 and To 5

We present the broad band Johnson U BV and Cousins RI CCD photometric observations for open clusters Be 10, Be 67 and To 5. So far no photometric study of these clusters is published. The colour-magnitude diagrams indicate that all the three clusters are of intermediate age. The reddening E0B-V), estimated from colour-colour diagrams/ colour-magnitude diagrams, is 0.87±0.05 mag for Be 10, 0.79±0.005 mag for Be 67 and 0.80±0.05 mag for To 5. The comparison of colour magnitude diagrams with the stellar models with convective overshoot by Girardi et al. (2002) produces a good fit for a metallicity Z=0.008 for cluster Be 10 and Z=0.02 for clusters Be 67 and To 5. Ages for Be 10, Be 67 and To 5 are log (ag) = 8.8±0.1, 9.0±0.1 and 8.3±0.2 respectively. The corresponding distances for these clusters are 2.29±0.21, 2.45±0.23 and 1.75±0.16 kpc respectively. Analysis of the radial distribution of stellar surface density indicates that radius values for Be 10, Be 67 and To 5 are 6.0, 3.5 and 6.0 arcmin respectively

Pre-main sequence variable stars in young open cluster NGC 1893

We present results of multi-epoch (fourteen nights during 2007-2010) $V$-band photometry of the cluster NGC 1893 region to identify photometric variable stars in the cluster. The study identified a total of 53 stars showing photometric variability. The members associated with the region are identified on the basis of spectral energy distribution, $J-H/H-K$ two colour diagram and $V/V-I$ colour-magnitude diagram. The ages and masses of the majority of pre-main-sequence sources are found to be $\lesssim$ 5 Myr and in the range 0.5 $\lesssim$ $M/M_{\odot}$ $\lesssim$ 4, respectively. These pre-main-sequence sources hence could be T Tauri stars. We also determined the physical parameters like disk mass and accretion rate from the spectral energy distribution of these T Tauri stars. The periods of majority of the T Tauri stars range from 0.1 to 20 day. The brightness of Classical T Tauri stars is found to vary with larger amplitude in comparison to Weak line T Tauri stars. It is found that the amplitude decreases with increase in mass, which could be due to the dispersal of disks of massive stars.Comment: 15pages, 16 figures and 5 tables, Accepted for publication in MNRA

Plasmodium vivax lineages: geographical distribution, tandem repeat polymorphism, and phylogenetic relationship

Background: Multi-drug resistance and severe/ complicated cases are the emerging phenotypes of vivax malaria, which may deteriorate current anti-malarial control measures. The emergence of these phenotypes could be associated with either of the two Plasmodium vivax lineages. The two lineages had been categorized as Old World and New World, based on geographical sub-division and genetic and phenotypical markers. This study revisited the lineage hypothesis of P. vivax by typing the distribution of lineages among global isolates and evaluated their genetic relatedness using a panel of new mini-satellite markers. Methods: 18S SSU rRNA S-type gene was amplified from 420 Plasmodium vivax field isolates collected from different geographical regions of India, Thailand and Colombia as well as four strains each of P. vivax originating from Nicaragua, Panama, Thailand (Pak Chang), and Vietnam (ONG). A mini-satellite marker panel was then developed to understand the population genetic parameters and tested on a sample subset of both lineages. Results: 18S SSU rRNA S-type gene typing revealed the distribution of both lineages (Old World and New World) in all geographical regions. However, distribution of Plasmodium vivax lineages was highly variable in every geographical region. The lack of geographical sub-division between lineages suggests that both lineages are globally distributed. Ten mini-satellites were scanned from the P. vivax genome sequence; these tandem repeats were located in eight of the chromosomes. Mini-satellites revealed substantial allelic diversity (7-21, AE = 14.6 +/- 2.0) and heterozygosity (He = 0.697-0.924, AE = 0.857 +/- 0.033) per locus. Mini-satellite comparison between the two lineages revealed high but similar pattern of genetic diversity, allele frequency, and high degree of allele sharing. A Neighbour-Joining phylogenetic tree derived from genetic distance data obtained from ten mini-satellites also placed both lineages together in every cluster. Conclusions: The global lineage distribution, lack of genetic distance, similar pattern of genetic diversity, and allele sharing strongly suggested that both lineages are a single species and thus new emerging phenotypes associated with vivax malaria could not be clearly classified as belonging to a particular lineage on basis of their geographical origin